| Serie 1 |
| Ximena Vasquez Rodriguez | Team Chile | 4 | 57.82 (CR) | 1 q |
| Daniela Alejandra Salazar Burga | Atletismo Chiclayo | 5 | 1:01.32 | 2 q |
| Shantall Sammy Gonzales Gutierrez | Féniz | 3 | 1:03.28 | 5 q |
| Yagaira Prisila Ordinola Ramos | Del Norte | 2 | DNS | |
| Belen Sofia Salinas Chocano | Panteras - Surco | 6 | DNS | |
| Alessandra Isabela Valdivia Jauregui | Academia de Barranco | 7 | DNS | |
| Luisa Gabriela Chacon Arana | Féniz | 8 | DNS | |
| Serie 2 |
| Catalina Izaga Mannuci | Regatas | 4 | 1:01.37 | 3 q |
| María Esmeralda Solano Rojas | Atletic Deportmanu | 5 | 1:04.67 | 9 |
| Hilary Jesbell Sanchez Gonzales | Atlético Ilbamir | 3 | 1:08.08 | 15 |
| Rafaela Luana Gutierrez Orellana | Féniz | 7 | 1:08.37 | 16 |
| Ivana Padme Llerena Limazca | IED FAP Raúl Leguia Drago | 8 | 1:12.91 | 19 |
| Amelia Aracely Correa Quiroz | Deportivo Los Gavilanes | 2 | DNS | |
| Rafaela Higa Suejiro | Atletismo Chiclayo | 6 | DNS | |
| Serie 3 |
| Nadja Valentina Landeo Castro | Alborada Track and Field | 4 | 1:02.28 | 4 q |
| Mariana Sofia Escobedo Vera | IED San Jerónimo | 6 | 1:03.86 | 7 q |
| Fabiana Carrera Segura | Regatas | 8 | 1:04.23 | 8 q |
| Bridge Dayana Enriquez Carcosto | Deportivo Athletic Puno Runners | 7 | 1:06.83 | 12 |
| Korina Collas Canario | Team Chile | 5 | 1:07.02 | 13 |
| Bianca Valeria Del Aguila Melgarejo | Atlético Ilbamir | 3 | 1:10.65 | 17 |
| Serie 4 |
| Alisson Leticia Nizama Valdiviezo | Olympic Tumbes | 4 | 1:03.70 | 6 q |
| Fatima Sofia Gutierrez Congona | Athletic Team AQP Sac | 8 | 1:06.39 | 10 |
| Karina Valdeiglesias Tinta | Deportivo Chasquis de Sicuani | 7 | 1:06.59 | 11 |
| Brenda Kamila Fernandez Guevara | Alborada Track and Field | 5 | 1:07.93 | 14 |
| Luciana Mariel Mogrovejo Galindo | Atlético Ilbamir | 3 | 1:11.23 | 18 |
| Valeria Santillán Chinchayán | Desarrollo Deportivo SAC | 6 | DNS | |
